Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: p16INK4a downregulation alleviates temporomandibular joint osteoarthritis combined with type 2 diabetes by driving M2 polarization.
doi: 10.1016/j.biopha.2025.118172
Figure Lengend Snippet: Fig. 7. MS37452 and P16KD work by promoting mitochondrial function and decreasing iron accumulation. (A) Intracellular Fe2+ levels were evaluated with FerroOrange fluorescent probe. (B) Detection of Fe2+, GSH, and MDA levels in M0 from different groups (Relative Fe2+ expression: Ctrl: M0 VS TNFα+GP: t = 12.31, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 11.67, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 12.82, df = 4. Relative GSH expression: Ctrl: M0 VS TNFα+GP: t = 11.52, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 25.95, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 10.19, df = 4. Relative MDA expression: Ctrl: M0 VS TNFα+GP: t = 18.61, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 32.29, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 33.10, df = 4.). (C) Western blot analysis of FTH1 and TFRC in M0 from different groups. (D) Co-IP was used to determine the interaction between p16 and GSH. Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages. GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indicated columns.
Article Snippet: After that, membranes were incubated at 4 ◦C overnight with primary antibodies against inducible nitric oxide synthase (INOS) (Proteintech Group, Rosemont, IL, USA), arginase 1 (Arg1) (Proteintech Group, Rosemont, IL, USA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Rosemont, IL, USA), transferrin receptor (TFRC) (Proteintech Group, Rosemont, IL, USA), vascular endothelial growth factor A (VEGFA) (Proteintech Group, Rosemont, IL, USA), alkaline phosphatase (ALP) (Proteintech Group, Rosemont, IL, USA), runt-related transcription factor 2 (RUNX2) (Proteintech Group, Rosemont, IL, USA), cluster differentiation 31 (CD31) (Proteintech Group, Rosemont, IL, USA), ferritin heavy chain (FTH1) (Proteintech Group, Rosemont, IL, USA).
Techniques: Expressing, Western Blot, Co-Immunoprecipitation Assay, Knockdown, Control, Derivative Assay
